Fillinger Sabine

Sabine Fillinger

Directrice de Recherches (Ph.D, HDR)


UR1290 BIOGER-CPP (bât 13)
Avenue Lucien Brétignières
F-78850 Thiverval-Grignon, France


Twitter @FillingerS512

phone: +33-1-3081-4565


My research aims at deciphering the mechanisms that plant pathogenic fungi develop to adapt (and resist!) to fungicides. The fungal models I work with are Botrytis cinerea (grey mould agent) and Zymoseptoria tritici (septoria leaf blotch).

My activity concerns the identification of resistance mechanisms using genetic and genomic approaches. I apply reverse genetics to validate mutations responsible for fungicide resistance, but also to study the resistance cost on fungal fitness.

Curriculum vitae



MSc & PhD Transcriptional regulation in the filamentous fungus Aspergillus nidulans (B. Felenbok) University Paris XI, France.


Postdoc “Systematic functional analysis of Bacillus subtilis genes, C-metabolism”(S. Aymerich)INRA INA-PG, Grignon, France.


Postdoc “Role of polyols and cAMP in germination in Aspergillus nidulans” (C. d’Enfert) Institut Pasteur, Paris, France


Senior scientist at INRA, Versailles-Grignon

-     Genetics of fungicide resistance in filamentous fungi.

-     Functional genomics of signal transduction in Botrytis cinerea

Since 2014

Research Director at INRA, Versailles-Grignon

-     Group leader of the “fungicide” research team

-     Associate head of the BIOGER department.

PhD students:

Weiwei Liu (2004-2008), Anaïs Lalève (2009-2012), Saad Azeddine (2010-2014), Jaafar Kilani (2014-2018), Yosra Ben M’henni (2015-2019), Guillaume Fouché (since 2018).

Editorial & expert activities:

  • Editor of “Botrytis –the Fungus, the Pathogen and its Management in Agricultural Systems” (2015), S. Fillinger & Y. Elad (eds.), Springer books, 596 pp.; Reviewer for microbiological and phytopathological journals.
  • Research Topic editor for Frontiers in Microbiology “Antifungals used against animal and plant pathogenic fungi"
  • Evaluation of scientific projects: BARD, Wellcome Trust, EU MSCA program, Expert for AERES/HCERES-France, Professor and Ass. Prof recruitment committees, INRA internal evaluation committees



Omrane, S., C. Audéon, A. Ignace, C. Duplaix, L. Aouini, G. Kema, A.-S. Walker & S. Fillinger, (2017) Plasticity of the MFS1 Promoter Leads to Multidrug Resistance in the Wheat Pathogen Zymoseptoria tritici. mSphere 2: pii: e00393-00317


Mair, W., F.L. Ruiz, G. Stammler, W. Clark, F. Burnett, D. Hollomon, H. Ishii, T.S. Thind, J.K.M. Brown, B. Fraaije, H. Cools, M. Shaw, S. Fillinger, E.M. Terrado, G. Schnabel, A. Mehl & R.P. Oliver, (2016) Proposal for a unified nomenclature for target site mutations associated with resistance to fungicides. Pest Manag Sci: in press. doi :10.1002/ps.4301

Fillinger, S. & Y. Elad, (2016) Botrytis - the Fungus, the Pathogen and its Management in Agricultural Systems, p. 486. Springer International Publishing, Cham, Heidelberg, New York, Dordrecht, London.

Fillinger, S. & A.-S. Walker, (2016) Chemical Control and Resistance Management of Botrytis Diseases. In: Botrytis – the Fungus, the Pathogen and its Management in Agricultural Systems. S. Fillinger & Y. Elad (eds). Cham: Springer International Publishing, pp. 189-216.


Omrane, S., H. Sghyer, C. Audéon, C. Lanen, C. Duplaix, A.-S. Walker & S. Fillinger, (2015) Fungicide efflux and the MgMFS1 transporter contribute to the multidrug resistance phenotype in Zymoseptoria tritici field isolates. Environmental microbiology 17: 2805-2823.


Laleve, A., S. Gamet, A.S. Walker, D. Debieu, V. Toquin & S. Fillinger, (2014) Site-directed mutagenesis of the P225, N230 and H272 residues of succinate dehydrogenase subunit B from Botrytis cinerea highlights different roles in enzyme activity and inhibitor binding. Environmental microbiology 16: 2253-2266.

Laleve, A., S. Fillinger & A.S. Walker, (2014) Fitness measurement reveals contrasting costs in homologous recombinant mutants of Botrytis cinerea resistant to succinate dehydrogenase inhibitors. Fungal Genet Biol 67: 24-36.

Davanture, M., J. Dumur, N. Bataillé-Simoneau, C. Campion, B. Valot, M. Zivy, P. Simoneau & S. Fillinger, (2014) Phosphoproteome profiles of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea during exponential growth in axenic cultures. PROTEOMICS 14: 1639-1645.


Debieu, D., J. Bach, E. Montesinos, S. Fillinger & P. Leroux, (2013) Role of sterol 3-ketoreductase sensitivity in susceptibility to the fungicide fenhexamid in Botrytis cinerea and other phytopathogenic fungi. Pest Manag Sci 69: 642-651.

Schumacher, J., A.l. Gautier, G. Morgant, L. Studt, P.-H. Ducrot, P. Le Pècheur, S. Azeddine, S. Fillinger, P. Leroux, B. Tudzynski & M. Viaud, (2013) A Functional Bikaverin Biosynthesis Gene Cluster in Rare Strains of Botrytis cinerea Is Positively Controlled by VELVET . PLoS ONE 8: e53729.


Billard, A., V. Laval, S. Fillinger, P. Leroux, H. Lachaise, R. Beffa & D. Debieu, (2012) The allele-specific probe and primer amplification assay, a new real-time PCR method for fine quantification of single-nucleotide polymorphisms in pooled DNA. Appl Environ Microbiol 78: 1063-1068.

Billard, A., S. Fillinger, P. Leroux, H. Lachaise, R. Beffa & D. Debieu, (2012) Strong resistance to the fungicide fenhexamid entails a fitness cost in Botrytis cinerea, as shown by comparisons of isogenic strains. Pest Manag Sci 68: 684-691.

Fillinger, S., S. Ajouz, P.C. Nicot, P. Leroux & M. Bardin, (2012) Functional and Structural Comparison of Pyrrolnitrin- and Iprodione-Induced Modifications in the Class III Histidine-Kinase Bos1 of Botrytis cinerea. PLoS ONE 7: e42520.


Mernke, D., S. Dahm, A.S. Walker, A. Lalève, S. Fillinger, M. Leroch & M. Hahn, (2011) Two Promoter Rearrangements in a Drug Efflux Transporter Gene Are Responsible for the Appearance and Spread of Multidrug Resistance Phenotype MDR2 in Botrytis cinerea Isolates in French and German Vineyards. Phytopathology 101: 1176-1183.

Liu, W., M.C. Soulie, C. Perrino & S. Fillinger, (2011) The osmosensing signal transduction pathway from Botrytis cinerea regulates cell wall integrity and MAP kinase pathways control melanin biosynthesis with influence of light. Fungal Genet Biol 48: 377-387.

Billard, A., S. Fillinger, P. Leroux, J. Bach, C. Lanen, H. Lachaise, R. Beffa & D. Debieu, (2011) Fenhexamid Resistance in the Botrytis Species Complex, Responsible for Grey Mould Disease. In: Fungicides - Beneficial and Harmful Aspects. N. Thajuddin (ed). InTech, pp. 61-78.

Amselem, J., C.A. Cuomo, J.A.L. van Kan, M. Viaud, E.P. Benito, A. Couloux, P.M. Coutinho, R.P. de Vries, P.S. Dyer, S. Fillinger, …, M.-H. Lebrun & M. Dickman, (2011) Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genet 7: e1002230.


Kretschmer, M., M. Leroch, A. Mosbach, A.S. Walker, S. Fillinger, D. Mernke, H.J. Schoonbeek, J.M. Pradier, P. Leroux, M.A. De Waard & M. Hahn, (2009) Fungicide-driven evolution and molecular basis of multidrug resistance in field populations of the grey mould fungus Botrytis cinerea. PLoS Pathog 5: e1000696.

Liu, W., P. Leroux & S. Fillinger, (2008) The HOG1-like MAP kinase Sak1 of Botrytis cinerea is negatively regulated by the upstream histidine kinase Bos1 and is not involved in dicarboximide- and phenylpyrrole-resistance. Fungal Genet Biol 45: 1062-1074.

Fillinger, S., P. Leroux, C. Auclair, C. Barreau, C. Al Hajj & D. Debieu, (2008) Genetic analysis of fenhexamid resistant field isolates of the phytopathogenic fungus Botrytis cinerea. Antimicrob Agents Chemother 52: 3933-3940.

Viaud, M., S. Fillinger, W. Liu, J.S. Polepalli, P. Le Pecheur, A.R. Kunduru, P. Leroux & L. Legendre, (2006) A class III histidine kinase acts as a novel virulence factor in Botrytis cinerea. Mol Plant Microbe In 19: 1042-1050.

Viaud, M., F. Legeai, J.M. Pradier, Y. Brygoo, F. Bitton, J. Weissenbach, A. Brunet-Simon, A. Duclert, S. Fillinger, D. Fortini, A. Gioti, C. Giraud, S. Halary, I. Lebrun, P. Le Pecheur, D. Samson & C. Levis, (2005) Expressed sequence tags from the phytopathogenic fungus Botrytis cinerea. Eur J Plant Pathol 111: 139-146.


le Coq, D., S. Fillinger & S. Aymerich, (1999) Histidinol phosphate phosphatase, catalyzing the penultimate step of the histidine biosynthesis pathway, is encoded by ytvP (hisJ) in Bacillus subtilis. J Bacteriol 181: 3277-3280.

Fillinger, S., C. Panozzo, M. Mathieu & B. Felenbok, (1995) The basal level of transcription of the alc genes in the ethanol regulon in Aspergillus nidulans is controlled both by the specific transactivator AlcR and the general carbon catabolite repressor CreA. FEBS Lett 368: 547-550.

Fillinger, S. & B. Felenbok, (1996) A newly identified gene cluster in Aspergillus nidulans comprises five novel genes localized in the alc region that are controlled both by the specific transactivator AlcR and the general carbon-catabolite repressor CreA. Mol Microbiol 20: 475-488.

Fillinger, S., S. Boschi-Muller, S. Azza, E. Dervyn, G. Branlant & S. Aymerich, (2000) Two glyceraldehyde-3-phosphate dehydrogenases with opposite physiological roles in a nonphotosynthetic bacterium. J Biol Chem 275: 14031-14037.

Fillinger, S., G. Ruijter, M.J. Tamas, J. Visser, J.M. Thevelein & C. d'Enfert, (2001) Molecular and physiological characterization of the NAD-dependent glycerol 3-phosphate dehydrogenase in the filamentous fungus Aspergillus nidulans. Mol Microbiol 39: 145-157.

Fillinger, S., M.K. Chaveroche, P. van Dijck, R. de Vries, G. Ruijter, J. Thevelein & C. d'Enfert, (2001) Trehalose is required for the acquisition of tolerance to a variety of stresses in the filamentous fungus Aspergillus nidulans. Microbiology 147: 1851-1862.

Fillinger, S., M.K. Chaveroche, K. Shimizu, N. Keller & C. d'Enfert, (2002) cAMP and ras signalling independently control spore germination in the filamentous fungus Aspergillus nidulans. Mol Microbiol 44: 1001-1016.

Kobayashi, K., S.D. Ehrlich, A. Albertini, G. Amati, K.K. Andersen, M. Arnaud, K. Asai, S. Ashikaga, S. Aymerich, P. Bessieres, F. Boland, S.C. Brignell, S. Bron, K. Bunai, J. Chapuis, L.C. Christiansen, A. Danchin, M. Debarbouille, E. Dervyn, E. Deuerling, K. Devine, S.K. Devine, O. Dreesen, J. Errington, S. Fillinger, …, U. Zuber & N. Ogasawara, (2003) Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100: 4678-4683.

Meima, R., C. Eschevins, S. Fillinger, A. Bolhuis, L.W. Hamoen, R. Dorenbos, W.J. Quax, J.M. van Dijl, R. Provvedi, I. Chen, D. Dubnau & S. Bron, (2002) The bdbDC operon of Bacillus subtilis encodes thiol-disulfide oxidoreductases required for competence development. J Biol Chem 277: 6994-7001.

Mathieu, M., S. Fillinger & B. Felenbok, (2000) In vivo studies of upstream regulatory cis-acting elements of the alcR gene encoding the transactivator of the ethanol regulon in Aspergillus nidulans. Mol Microbiol 36: 123-131.

Panozzo, C., V. Capuano, S. Fillinger & B. Felenbok, (1997) The zinc binuclear cluster activator AlcR is able to bind to single sites but requires multiple repeated sites for synergistic activation of the alcA gene in Aspergillus nidulans. J Biol Chem 272: 22859-22865.

de Vries, R.P., S.J. Flitter, P.J. van de Vondervoort, M.K. Chaveroche, T. Fontaine, S. Fillinger, G.J. Ruijter, C. d'Enfert & J. Visser, (2003) Glycerol dehydrogenase, encoded by gldB is essential for osmotolerance in Aspergillus nidulans. Mol Microbiol 49: 131-141.

Date de modification : 03 juillet 2023 | Date de création : 01 février 2012 | Rédaction : Sabine Fillinger